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KMID : 0545120210310121722
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Journal of Microbiology and Biotechnology
2021 Volume.31 No. 12 p.1722 ~ p.1731
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An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces
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Dong Jianxin
Wei Jiaxiu
Li Han
Zhao Shiyao
Guan Wenjun
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Abstract
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The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.
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KEYWORD
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Industrial Streptomyces, indigoidine synthetase, large DNA fragment deletion, biosynthetic gene cluster
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